Analysis Note

ControlHeLa cell lysate.

Application

Research Sub CategoryCytoskeleton

This Anti-SDHD (CybS) Antibody is validated for use in Western Blotting, ICC for the detection of SDHD (CybS).

Research CategoryCell Structure

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SDHD (CybS) in NIH/3T3 and HeLa cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Succinate dehydrogenase complex subunit D (SDHD) is a member of the CybS family and is one of four subunits of the succinate dehydrogenase (SDH) complex involved in the electron transport chain of the mitochondria. The specific role of SDH is to transfer electrons from succinate to ubiquinone, and the SDHD subunit is involved in anchoring the SDH complex to the inner mitochondrial membrane. Defective SDHD has been linked to Carney-Stratakis syndrome, a condition characterized by concurrent paraganglioma and gastric stromal sarcomas.

Immunogen

Epitope: Topological domain (mitochondrial matrix)

KLH-conjugated linear peptide corresponding to the Topological domain of human SDHD (CybS).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SDHD (CybS) in 10 µg of HeLa cell lysate.

Specificity

Other homologies: Rat (92% sequence homology).

This antibody recognizes the Topological domain (mitochondrial matrix) of SDHD (CybS).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~11 kDa observed. An uncharacterized band may be observed at ~25 kDa in some cell lysates.